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human proteins β actin c4  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology human proteins β actin c4
    EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.
    Human Proteins β Actin C4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 46609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+proteins+%CE%B2+actin+c4/%CE%B2-Actin+Antibody/pmc05451862-69-8-26
    Average 96 stars, based on 46609 article reviews
    human proteins β actin c4 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs"

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

    Journal: Disease Markers

    doi: 10.1155/2017/9653194

    EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.
    Figure Legend Snippet: EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.

    Techniques Used: Isolation, Western Blot, Negative Control, Control, Molecular Weight

    Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.
    Figure Legend Snippet: Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

    Techniques Used: Isolation, Western Blot, Negative Control, Control, Zymography, Molecular Weight

    Related Articles

    Isolation:

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs
    Article Snippet: Results were given as the protein to β -actin ratio.Results were given as the protein to β -actin ratio.. The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).. All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.

    Western Blot:

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs
    Article Snippet: Results were given as the protein to β -actin ratio.Results were given as the protein to β -actin ratio.. The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).. All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.

    Negative Control:

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs
    Article Snippet: Results were given as the protein to β -actin ratio.Results were given as the protein to β -actin ratio.. The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).. All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.

    Control:

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs
    Article Snippet: Results were given as the protein to β -actin ratio.Results were given as the protein to β -actin ratio.. The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).. All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.

    Molecular Weight:

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs
    Article Snippet: Results were given as the protein to β -actin ratio.Results were given as the protein to β -actin ratio.. The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).. All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.

    Zymography:

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs
    Article Snippet: Results were given as the protein to β -actin ratio.Results were given as the protein to β -actin ratio.. The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).. All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.All primary antibodies were used in a 1 : 50 dilution; only anti-FN1-EDA was diluted 1 : 500.



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    EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.
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    Image Search Results


    EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.

    Journal: Disease Markers

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

    doi: 10.1155/2017/9653194

    Figure Lengend Snippet: EV isolation from ascites. EVs were isolated from ascites with the ligands AV (lane 1), CTB (lane 2), and STB (lane 3) and analyzed by western blotting. As a negative control, no ligand was added (lane 4). 250 μ l of pooled ascites from 6 (a) or 16 (b) ovarian cancer patients were used as starting material. The tetraspanin proteins CD9 and CD63, the transferrin receptor (CD71), and the cytosolic protein Alix were examined, and β -actin was used as a loading control. The normalized band intensities of the analyzed proteins are given below each band. The molecular weight markers are indicated on the left side of the images in kDa.

    Article Snippet: The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).

    Techniques: Isolation, Western Blot, Negative Control, Control, Molecular Weight

    Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

    Journal: Disease Markers

    Article Title: EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

    doi: 10.1155/2017/9653194

    Figure Lengend Snippet: Cancer proteins in EV isolations. (a) EVs isolated from 250 μ l of pooled ascites from 6 ovarian cancer patients (lanes 1–4) or from 5 patients with cirrhosis (lanes 5–8) were analyzed by western blot. As before the ligands AV, CTB, and STB were used for isolation, no ligand was added as a negative control. The protein cellular fibronectin (FN1-EDA), CD59, β -catenin, and epithelial cell adhesion molecule (EpCAM) were examined, and β -actin was used as loading control. The normalized band intensities of the analyzed proteins are given below each band. (b) EVs isolated from 500 μ l of the same samples as described for (a) were analyzed by zymography. The left part of the image (lanes 1–5) shows the ovarian cancer samples and the right part the cirrhosis samples (lanes 6–10). In lanes 1 and 6, 1 μ l of the pooled ascites samples were analyzed. The molecular weight markers are indicated on the left side of the images in kDa.

    Article Snippet: The primary antibodies were mouse antibodies against the human proteins β -actin (C4), β -catenin (E-5), CD71 (H68.4), CD9 (C-4), Alix (1A12), CD59 (H-7), EpCAM (0.N.276) (Santa Cruz Biotechnology Inc., USA), CD63 (H5C6) (BD Biosciences, USA), and FN1-EDA (IST-9) (abcam PLC, UK).

    Techniques: Isolation, Western Blot, Negative Control, Control, Zymography, Molecular Weight